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Category: Workflow

Type: Galaxy

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Galaxy

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Marc Ciosi

cutadapt+BWA-MEM-mis1-i2,2-gapex2,2+filter... (1)

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This workflow is designed to genotype trinucleotide repeats from sequencing reads that span the repeat and its flanksDetailed description as follows:1. Input fastq sequencing reads files (Galaxy tool: Input dataset)Input files for the workflow are single-end MiSeq reads (R1 produced using the protocol from Ciosi et al. 2018) or PacBio reads of insert (ROI) in fastq files format.2. Removing Illumina sequencing adaptor sequence in 3’ of the MiSeq reads (Galaxy tool: Cutadapt 1.16)Cutadapt...

Created: 2020-06-01 | Last updated: 2020-06-01

Galaxy

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Vilija Lomeikaite

Human MSH3 exon 1 variant genotyping (1)

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This workflow is designed to genotype 9 bp tandem repeat region in MSH3 exon 1 as well as flanking variants described in Flower and Lomeikaite et al. (2018). Detailed description as follows:1. Input files.Input files for the workflow are demultiplexed fastq files from MiSeq Illumina sequencing. 2. Read merging.To enable obtaining haplotype information, forward and reverse paired-end reads are merged using Pear with default settings. 3. Demultiplexing.Merged reads are demul...

Created: 2018-08-22 | Last updated: 2018-08-22

Galaxy

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Christophe Antoniewski

Metavisitor: Spades test in Use Case 2-2 (1)

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Metavisitor, a suite of Galaxy tools for simple and rapid detection and discovery of viruses in deep sequence dataReadthedocMississippi Galaxy server

Created: 2016-10-10 | Last updated: 2016-10-10

Galaxy

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Christophe Antoniewski

Metavisitor: Trinity test in Use Case 2-2 (1)

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Metavisitor, a suite of Galaxy tools for simple and rapid detection and discovery of viruses in deep sequence dataReadthedocMississippi Galaxy server

Created: 2016-10-10 | Last updated: 2016-10-10

Galaxy

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Christophe Antoniewski

Metavisitor: Workflow for small RNA profil... (1)

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Metavisitor, a suite of Galaxy tools for simple and rapid detection and discovery of viruses in deep sequence dataReadthedocMississippi Galaxy server

Created: 2016-10-10 | Last updated: 2016-10-10

Galaxy

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Y. Hoogstrate

VCaP RNA-Seq Fusion gene detection with ST... (1)

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The end-to-end workflow starts by obtaining paired-end RNA-Seq data of the VCaP cell line via EGA. Galaxy determines the file type automatically with its built-in format detection system. The reads will be clipped before an alignment with STAR and STAR produces several output files that can be used for different analysis. The last tool is STAR-Fusion which was able to confirm the TMPRSS2-ERG fusion gene.