<?xml version="1.0" encoding="UTF-8"?>
<!--WARNING: Do not change anything inside this workflow file. BioExtract won't recognize your changes, or may even refuse to import it. Instead, modify the workflow in BioExtract after importing. This workflow file can be imported into BioExtract only.-->
  <s:bioextract xmlns:s="http://bioextract.org" version="2.1" log="0">
<s:workflowdescription description="This workflow retreive known SNP located on the human chromosome 8 between positions 148200 and 148800 then propose Primers and Restriction enzymes taht can be used within the same region. The same workflow can be used for any other region in the Human genome." author="yosr.bouhlal@gmail.com" uid="b0789898-ac96-4ba2-a3b4-a3f4005adfe9" title="Biomart SNP"/>
<s:processor name="BioMart Query"><s:description>""</s:description><s:helpurl>""</s:helpurl></s:processor><s:processor name="BioMart Query"><s:description>""</s:description><s:helpurl>""</s:helpurl></s:processor><s:processor name="eprimer3"><s:description>"Picks PCR primers and hybridization oligos"</s:description><s:soaplabwsdl>http://www.ebi.ac.uk/soaplab/services/nucleic_primers.eprimer3</s:soaplabwsdl><s:helpurl>"http://emboss.sourceforge.net/apps/release/6.1/emboss/apps/eprimer3.html"</s:helpurl><s:parameter name="task" value="1"/><s:parameter name="numreturn" value="5"/><s:parameter name="gcclamp" value="0"/><s:parameter name="osize" value="20"/><s:parameter name="minsize" value="18"/><s:parameter name="maxsize" value="27"/><s:parameter name="otm" value="60.0"/><s:parameter name="mintm" value="57.0"/><s:parameter name="maxtm" value="63.0"/><s:parameter name="maxdifftm" value="100.0"/><s:parameter name="ogcpercent" value="50.0"/><s:parameter name="mingc" value="20.0"/><s:parameter name="maxgc" value="80.0"/><s:parameter name="saltconc" value="50.0"/><s:parameter name="dnaconc" value="50.0"/><s:parameter name="maxpolyx" value="5"/><s:parameter name="psizeopt" value="200"/><s:parameter name="prange" value="100-300"/><s:parameter name="ptmopt" value="0.0"/><s:parameter name="ptmmin" value="-1000000.0"/><s:parameter name="ptmmax" value="1000000.0"/><s:parameter name="osizeopt" value="20"/><s:parameter name="ominsize" value="18"/><s:parameter name="omaxsize" value="27"/><s:parameter name="otmopt" value="60.0"/><s:parameter name="otmmin" value="57.0"/><s:parameter name="otmmax" value="63.0"/><s:parameter name="ogcopt" value="50.0"/><s:parameter name="ogcmin" value="20.0"/><s:parameter name="ogcmax" value="80.0"/><s:parameter name="osaltconc" value="50.0"/><s:parameter name="odnaconc" value="50.0"/><s:parameter name="oanyself" value="12.00"/><s:parameter name="oendself" value="12.00"/><s:parameter name="opolyxmax" value="5"/><s:parameter name="omishybmax" value="12.0"/><s:parameter name="firstbaseindex" value="1"/><s:parameter name="maxmispriming" value="12.00"/><s:parameter name="pairmaxmispriming" value="24.00"/><s:parameter name="numnsaccepted" value="0"/><s:parameter name="selfany" value="8.00"/><s:parameter name="selfend" value="3.00"/><s:parameter name="maxendstability" value="9.0"/></s:processor><s:processor name="FormatConversion"><s:description>"Convert from one allowed format to another allowed format"</s:description><s:helpurl>"http://emboss.sourceforge.net/docs/themes/SequenceFormats.html"</s:helpurl><s:parameter name="To Format" value="plain"/><s:parameter name="From Format" value="fasta"/></s:processor><s:processor name="RestrictionMapper"><s:description>"RestrictionMapper"</s:description><s:helpurl>"http://bioservices.usd.edu/opal/services/RestrictionMapper_BioExtract"</s:helpurl><s:parameter name="Specifies DNA conformation" value="linear"/><s:parameter name="maximum number of cuts per enzyme" value="all"/><s:parameter name="minimum length of the recognition site" value="5"/><s:parameter name="Enzyme Type" value="all"/><s:parameter name="First sort field" value="frequency"/><s:parameter name="Second sort field" value="overhang"/><s:parameter name="Third sort field" value="name"/><s:parameter name="Map filename (tab delim)" value="RestrictionMapper_map.tab"/></s:processor><s:link source="query:value" sink="BioMart Query:sequence_usa" />
<s:link source="BioMart Query:outseq" sink="BioMart Query:sequences_direct_data" />
 <s:link source="BioMart Query:Graphics_in_PNG" sink="BioMart Query" />
<s:sink name="BioMart Query">
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  </s:bioextract>