In User: Drkrishnasarmapathy


Name: Drkrishnasarmapathy

Joined: Tuesday 24 July 2012 08:13:50 (UTC)

Last seen: Thursday 21 June 2018 05:33:28 (UTC)

Email (public): pathykdr [at]


Location: vadodara, India

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HPLC method development and validation play important role in the discovery, development and manufacture of   agro chemicals , pharmaceutical products. This article mainly focuses on the optimization of HPLC conditions and other important perspectives during method  development and validation. Various critical steps related to analytical method development and validation is discussed. A sequence of events required for method development and analytical

validation are described. The steps involved in developing a stability-indicating HPLC method influences the analysis of degradation products/impurities in stability study and its validation demonstrate the suitability for its intended purpose.High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques in industry. It is used to separate and analyse compounds through the mass-transfer of analytes between stationary and mobile phases1-3. The technique is employed in a broad range of activities, such as the analysis of foods, drugs and agrochemicals. The technique of HPLC utilises a liquid mobile phase to separate the components of a mixture. The components themselves are first dissolved in a solvent and then forced to flow (via the mobile phase) through a column (stationary phase) under highpressure. The mixture is resolved into its components within the column and the amount of resolution is dependent upon the interaction between the solute components and the column stationary phase (immobile packing within the column) and liquid phase. The interaction of the solute with the mobile and stationary phases can be manipulated through different choices of both solvent and stationary phases

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