Retention Time Prediction with X!Tandem
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This workflow identifies peptides from tandem mass spectra using X!Tandem as in a standard installation of the Trans-Proteomic Pipeline (TPP, version 4.6, but earlier versions should also work). The peptide-spectrum matches are validates using PeptideProphet (also from a standard installation of TPP) and peptides with at least a probability 0.95 are used to train a linear retention time predictor (Palmblad et al., 2002), whereby retention coefficients are also derived. These indirectly provid...
Created: 2012-09-03
| Last updated: 2012-09-03
Credits:
Magnus Palmblad
Yassene
Eleni
Attributions:
rt3.c
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