Blast_Align_and_Tree

Created: 2013-01-28 08:50:21      Last updated: 2013-01-30 12:18:08

This workflow accepts a protein sequence as input. This sequence is compared to others in the Uniprot database, using the NCBI BLAST Web Service from the EBI (WSDL), and the top 10 hits are returned (Nested workflow:EBI_NCBI_BLast). For each extracted hit, the Uniprot REST service returns the protein sequence in FASTA format. The workflow concatenates the 10 protein sequences and submits them as input to the EBI CLustalw service (Nested workflow EMBL_EBI_clustalw2_SOAP). These sequences are aligned and returned as results. Finally, the alignment is submitted to the EBI Clustalw_phylogeny service (Nested Workflow: clustalw_phylogeny), and a phylogenetic tree in phylip format is retuned. The workflow returned a list of protein sequences in FASTA format, a Clustalw alignment, and a phylogenetic tree.

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