HLA ligandomics workflow using OpenMS and ImmunoNodes

Created: 2016-11-30 15:23:38      Last updated: 2017-04-11 18:51:47

HLA ligandomics workflow combining native KNIME, OpenMS, and ImmunoNodes nodes. The workflow extracts MS data from PRIDE and performs mass spectra identification with X!Tandem, annotates the results with details of the given target/decoy database, calculates false discovery rates and filters for 5% FDR using OpenMS’ nodes. The identified peptides are annotated with their respective binding affinity predicted by NetMHC using the EpitopePrediction node. Finally, simple summary statistics and visualizations are generated with the use of native KNIME nodes

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