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Mapping OligoNucleotides to an assembly
(v7)
Created: 13/02/09 @ 09:05:35
| Last updated: 13/02/09 @ 09:08:20
Credits:
Wassinki
Pieter Neerincx
Attributions:
Blat against ENSEMBLE Danio_rerio_Genome
BlatBlastCombi
Blast against ENSEMBLE Danio_rerio_Genome
AppendToFile
Test Input for Mapping oligonucleotides to an assembly
Input for Mapping oligonucleotides to an assembly
License: Creative Commons Attribution-Share Alike 3.0 Unported License
Version info
The former version of the workflow expected that results from BioMART only report transcripts when the query (the probe in our
case) are entirely encapsulated in an exon of that transcript. However, the BioMart service also returns transcripts when the query is not or only partially overlapping with an exon in the stretch on the assembly on which a transcript is defined. This resulted in too many oligos classified as having multiple transcripts or having multiple genes.
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