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Arabidopsis thaliana QTL Analysis

Workflow Entry: Arabidopsis thaliana QTL Analysis

Created at: 08/07/09 @ 16:26:13 Last updated: 14/12/09 @ 11:59:14

Version 1 (of 1)

Version created on: 08/07/09 @ 16:26:13 by: Paul Fisher | Revision comments Expand

Last edited on: 14/12/09 @ 11:59:16 by: Paul Fisher

Title: Arabidopsis thaliana QTL Analysis

Type: Taverna 1

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Description

This workflow searches for genes which reside in a QTL (Quantitative Trait Loci) region in Arabidopsis thaliana. The workflow requires an input of: a chromosome name or number; a QTL start base pair position; QTL end base pair position. Data is then extracted from BioMart to annotate each of the genes found in this region. The UniProt identifiers are then sent to KEGG to obtain KEGG gene identifiers. The KEGG gene identifiers are then used to searcg for pathways in the KEGG pathway database.

Example input for this workflow are given below.

Chromosome_name: 5

start_position: 23446557

end_position: 26046557

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Option 1:

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Note: you need to have both the WHIP Launcher and the Taverna myExperiment/WHIP plugin installed on your machine for this to work. See here for information.

Option 2:

Copy and paste this link into File > 'Open workflow location...'
http://www.myexperiment.org/workflows/831/download?version=1
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Workflow Components

Inputs (3)

Name	Description
chromosome_name	This port requires a chromosome name, e.g. 17 or X
qtl_start_position	This port requires a chromosome or QTL start position in nucleotide base pairs, e.g. 28500000
qtl_end_position	This port requires a chromosome or QTL end position in nucleotide base pairs, e.g. 30500000

Processors (30)

Name	Type	Description
remove_pathway_duplicates	local
kegg_pathway_release	stringconstant
merge_pathway_list_2	local
merge_kegg_references	local
merge_gene_desc	local
split_for_duplicates	local
merge_uniprot_ids	local
remove_duplicate_kegg_genes	local
merge_genes_and_pathways	local
merge_genes_and_pathways_2	local
merge_genes_and_pathways_3	local
merge_pathway_list_1	local
merge_pathway_desc	local
regex_2	stringconstant
remove_uniprot_duplicates	local
remove_pathway_nulls	beanshell
split_gene_ids	beanshell
add_uniprot_to_string	beanshell
remove_pathway_nulls_2	beanshell
remove_nulls	beanshell
remove_nulls_3	beanshell
Kegg_gene_ids	arbitrarywsdl	See the Service entry for this web service.
gene_descriptions	arbitrarywsdl	See the Service entry for this web service.
binfo	arbitrarywsdl	See the Service entry for this web service.
Get_pathways	workflow	This workflow removes any null values from a list of strings
athaliana_qtl_region	biomart	Arabidopsis thaliana genes
athaliana_gene_ensembl	biomart	Arabidopsis thaliana genes
merge_pathway_descriptions	local
split_pathway_duplicates	local
remove_duplicate_pathway_ids	local

Beanshells (8)

Name	Inputs	Outputs
concat_ids	input	output
concat_gene_pathway_ids	pathway_ids gene_id	output
remove_pathway_nulls	input	output
split_gene_ids	input	output
add_uniprot_to_string	input	output
remove_pathway_nulls_2	input	output
remove_nulls	input	output
remove_nulls_3	input	output

Outputs (6)

Name	Description
merged_pathways
gene_descriptions
pathway_descriptions
kegg_pathway_release
kegg_external_gene_reference
pathway_ids

Links (38)

Source	Sink
Get_pathways:pathway_genes	merge_genes_and_pathways:stringlist
Get_pathways:pathway_ids	merge_pathway_list_1:stringlist
add_uniprot_to_string:output	Kegg_gene_ids:string
chromosome_name	athaliana_qtl_region:athaliana_gene_ensembl.chromosome_name_filter
gene_descriptions:return	merge_gene_desc:stringlist
kegg_pathway_release:value	binfo:db
merge_gene_desc:concatenated	remove_nulls_3:input
merge_genes_and_pathways:concatenated	merge_genes_and_pathways_2:stringlist
merge_genes_and_pathways_2:concatenated	merge_genes_and_pathways_3:stringlist
merge_pathway_desc:concatenated	remove_pathway_nulls:input
merge_pathway_list_2:concatenated	remove_pathway_nulls_2:input
qtl_end_position	athaliana_qtl_region:athaliana_gene_ensembl.end_filter
qtl_start_position	athaliana_qtl_region:athaliana_gene_ensembl.start_filter
Get_pathways:pathway_desc	merge_pathway_descriptions:stringlist
Kegg_gene_ids:return	merge_kegg_references:stringlist
Kegg_gene_ids:return	split_gene_ids:input
athaliana_gene_ensembl:athaliana_gene_ensembl.uniprot_swissprot	remove_uniprot_duplicates:stringlist
athaliana_qtl_region:athaliana_gene_ensembl.ensembl_gene_id	athaliana_gene_ensembl:athaliana_gene_ensembl.ensembl_gene_id_filter
merge_pathway_descriptions:concatenated	split_pathway_duplicates:string
merge_pathway_list_1:concatenated	remove_duplicate_pathway_ids:stringlist
regex_2:value	split_pathway_duplicates:regex
remove_duplicate_pathway_ids:strippedlist	merge_pathway_list_2:stringlist
split_pathway_duplicates:split	remove_pathway_duplicates:stringlist
binfo:return	kegg_pathway_release
merge_genes_and_pathways_3:concatenated	merged_pathways
merge_kegg_references:concatenated	kegg_external_gene_reference
merge_uniprot_ids:concatenated	remove_nulls:input
regex_2:value	split_for_duplicates:regex
remove_duplicate_kegg_genes:strippedlist	Get_pathways:gene_ids
remove_duplicate_kegg_genes:strippedlist	gene_descriptions:string
remove_nulls:output	add_uniprot_to_string:input
remove_nulls_3:output	gene_descriptions
remove_pathway_duplicates:strippedlist	merge_pathway_desc:stringlist
remove_pathway_nulls:output	pathway_descriptions
remove_pathway_nulls_2:output	pathway_ids
remove_uniprot_duplicates:strippedlist	merge_uniprot_ids:stringlist
split_for_duplicates:split	remove_duplicate_kegg_genes:stringlist
split_gene_ids:output	split_for_duplicates:string

Coordinations (0)

Information Workflow Type

Taverna 1

Information Original Uploader

Paul Fisher

Information License

All versions of this Workflow are licensed under:

Information Credits (1)

(People/Groups)

Paul Fisher

Information Attributions (0)

(Workflows/Files)

None

Information Tags (15)

Original Uploader tags

arabidopsis
|
chromosome
|
crop
|
ensembl
|
kegg
|
loci
|
locus
|
nucleotide
|
pathway
|
pathways
|
plants
|
qtl
|
thaliana
|
trait
|
uniprot

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Information Shared with Groups (1)

iPlantCollaborative

Information Featured In Packs (0)

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Information Attributed By (0)

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None

Information Favourited By (0)

No one

Information Statistics

638 viewings

594 downloads

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Citations (0)

None

Version History

Earliest Version:
[1] - Arabidopsis thaliana QTL Analysis

Created on: Wednesday 08 July 2009 @ 16:26:13 (GMT)

Created by: Paul Fisher

Last edited on: Monday 14 December 2009 @ 11:59:16 (GMT)

Last edited by: Paul Fisher

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