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Created: 03/10/07 @ 18:35:47 License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
Part of a workflow by Hannah Tipney, adapted by Duncan Hull using GenScan, RepeatMasker and BLAST.
http://dx.doi.org/10.1093/nar/gkl320
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Created: 03/10/07 @ 18:36:06 | Last updated: 03/12/09 @ 16:31:20 License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
Perform a sequence similarity search using the BLAST algorithm through the DDBJ web service.
Example input for this service are given below.
query:
>MySequence
MATDDSIIVLDDDDEDEAAAQPGPSNLPPNPASTGPGPGLSQQATGLSEPRVDGGSS
NSGSRKCYKLDNEKLFEEFLELCKTETSDHPEVVPFLHKLQQRAQSVFLASAEFCNIL
SRVLARSRKRPAKIYVYINELCTVLKAHSIKKKLNLAPAASTTSEASGPNPPTEPPSDLT
NTENTASEASRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSSYLQEAR
LKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGLDTFPDY
GDVLRAVEKAATRHSLGLP...
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Created: 03/10/07 @ 18:36:06 | Last updated: 03/12/09 @ 16:28:50 License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
Perform a blastp search on protein sequence and extract information based on the user input, e.g. a list of GI numbers. N.B. this workflow does not function correctly as it is designed for use with NCBI blast scripts. Some errors may occur. Please use two blast text file inputs for a secure result output.
Example input for this service are given below.
query:
>MySequence
MATDDSIIVLDDDDEDEAAAQPGPSNLPPNPASTGPGPGLSQQATGLSEPRVDGGSS
NSGSRKCYKLDNEKLFEEFLELCKTETSDHPEVVPFLHKLQQRAQSV...
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Created: 03/10/07 @ 18:36:06 | Last updated: 28/07/09 @ 13:01:45 License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
This workflow simplifies a BLAST text file into identifiers, descriptions and values (P, E-values). In order to extract the relevant ids etc. you need to pass the relevant string into the corresponding port, e.g. the default port being used is gi. This has been passed "gi". For any other ports simply pass in the string the SAME as the port name, e.g. seq_id, p, per etc.
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Created: 20/11/07 @ 16:58:38 | Last updated: 10/01/08 @ 12:09:38
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This is a workflow to automate multiple BLASTp jobs on a large list of protein sequences in FASTA format.
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Created: 09/01/08 @ 12:03:03 | Last updated: 09/01/08 @ 12:31:27
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow performs a generic protein sequence analysis. In order to do that a novel protein sequence enters into the software along with a list of known protein identifiers chosen by the biologist to perform a homology search, followed by a multiple sequence alignment and finally a phylogenetic analysis.
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Created: 17/06/09 @ 16:51:32
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License: Creative Commons Attribution 3.0 Unported License
This workflow retrieves a sequence associated with its features in embl format
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Created: 03/10/07 @ 18:36:12 | Last updated: 06/03/08 @ 17:01:32 License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
This workflow Performs a blastp search on protein sequence, extracts sequence id within the blast report and retrives the corresponding seuqences.
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Created: 30/07/08 @ 16:36:55 | Last updated: 03/12/09 @ 16:54:02
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieves a protein sequence in Fasta format from GenBank, given a GenBank identifier.
Example input for this workflow is:
EDL10223.1
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Created: 03/12/08 @ 14:17:20
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow is a GWorkflowDL representation of a sequence that sequentially invokes A and B.
This workflow is equivalent to the following pseudo code:
end_A = A();
end_B = B(end_A);
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Created: 09/03/09 @ 11:38:10
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow queries Biomart to retrieve the Ensembl gene id, protein id, gene name, description and amino acid sequence from the Ensembl Homo sapiens dataset. The user needs to specify a defined chromosomal region i.e. Chromo = 1, Start = 100000000, End = 250000000. This returns all unique entries in FASTA format.
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Created: 29/04/09 @ 16:39:57
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow generates DOI record files for deposit, using data set metadata for the FLOSSmole project. It reads in an input file generated from a SQL query from an eprints database, and transforms the parts of the source file as necessary to create a comprehensive DOI deposit record. It also generates DOIs for the data sets. These metadata are inserted into an XML record template (based on the std-doi.xsd schema) and the individual resources are aggregated into a single file.
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Created: 07/05/09 @ 14:28:59
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
To query protein sequence infomation out of 3 caGrid data services: caBIO, CPAS and GridPIR
Adapted from http://www.myexperiment.org/workflows/600
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Created: 05/04/10 @ 06:20:58
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow calculates and graphs the GC skew of a given genome sequence. Use this as a template for using more than 100 analysis programs implemented in G-language Genome Analysis Environment, which can be used in a similar manner. See http://www.g-language.org/ for more information about the G-language Genome Analysis Environment.
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Created: 05/04/10 @ 06:24:02
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow calculates and graphs the GC skew (by default, for keto bases, with window size of 1000) of a given genome sequence identifier. Here the genome sequence in Fasta format is downloaded through the Togo Web Service with RefSeq identifier. See http://www.g-language.org/ for more information about the G-language Genome Analysis Environment.
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Created: 05/04/10 @ 06:13:16 | Last updated: 05/04/10 @ 06:13:17
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow shows simple sequence manipulation functions of G-language GAE, such as random shuffling, obtaining a reverse complement, and translation of nucleotide sequences, and showing basic composition statistics for nucleotide and amino acid sequences. See http://www.g-language.org/ for more information about the G-language Genome Analysis Environment.
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Created: 05/04/10 @ 06:22:28
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow calculates and graphs the GC skew of a given genome sequence (for the entire genome, for only the coding sequences, for only the intergenic regions, and for only the third codon positions), as well as the GC content with sliding windows. See http://www.g-language.org/ for more information about the G-language Genome Analysis Environment.
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Created: 05/04/10 @ 06:17:25 | Last updated: 09/01/11 @ 06:08:19
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License: GNU General Public License (GPL) 2.0
This workflow calculates and graphs the AT skew of a given genome sequence, using options of gcskew program. See http://www.g-language.org/ for more information about the G-language Genome Analysis Environment.
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Created: 26/01/10 @ 14:46:07 | Last updated: 26/01/10 @ 14:46:08
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Perform an InterProScan analysis of a protein sequence using the EBI’s WSInterProScan service (see http://www.ebi.ac.uk/Tools/webservices/services/interproscan). The input sequence to use and the user e-mail address are inputs, the other parameters for the analysis (see Job_params) are allowed to default.
InterProScan searches a protein sequence against the protein family and domain signature databases integrated into InterPro (see http://www.ebi.ac.uk/interpro/). A set of matches to the sig...
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Created: 26/01/10 @ 14:45:46 | Last updated: 24/11/10 @ 10:04:09
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Perform an InterProScan analysis of a protein sequence using the EBI’s WSInterProScan service (see http://www.ebi.ac.uk/Tools/webservices/services/interproscan). The input sequence to use and the user e-mail address are inputs, the other parameters for the analysis (see Job_params) are allowed to default.
InterProScan searches a protein sequence against the protein family and domain signature databases integrated into InterPro (see http://www.ebi.ac.uk/interpro/). A set of matches to t...
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Created: 03/07/09 @ 14:18:28 | Last updated: 03/07/09 @ 14:19:32
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Using Biomart and EMBOSS soaplab services, This workflow retrieves a number of sequences from 3 species: mouse, human, rat; align them, and returns a plot of the alignment result. Corresponding sequence ids are also returned.
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Created: 10/07/09 @ 14:31:27 | Last updated: 30/11/09 @ 09:37:38
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License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
http://amc-app1.amc.sara.nl/twiki/bin/view/
Workflow-based DNA sequence analysis on the Dutch Life Science Grid,
presented as Application Showcase at the NBIC Conference 2009, Lunteren, The Netherlands, 17 & 18 March 2009.
http://www.biomedgrid.it/programme
may 15th 2009. Hands on workflow: grid-enabled medical imaging (Johan Montagnat – Tristan Glatard)
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Created: 20/11/09 @ 11:12:43 | Last updated: 30/11/09 @ 09:35:38
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
http://amc-app1.amc.sara.nl/twiki/bin/view/
Workflow-based DNA sequence analysis on the Dutch Life Science Grid.
This workflow is based on http://www.myexperiment.org/workflows/840 , the last component (Blast analysis) is replaced by Blat analysis
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Created: 19/03/10 @ 13:16:24 | Last updated: 19/03/10 @ 13:33:53
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Takes GI number for source (non-pathogenic) and target (pathogneic) genomes, extracts list of all proteins from each genome using GenBank database. Outputs prtoeins in FastA format. Creates database from source proteins using formatdb (locally installed) and blasts (local installed) proteins from target against this database. Extracts protens which are unique (no blast hits) to the target (pathogenic) genome based on eValue set by user. Takes unique proteins from target and blasts aga...
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Created: 19/03/10 @ 15:17:53
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieves the genome seqn for both the target and source strains using gi nos
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Created: 05/04/10 @ 06:08:25 | Last updated: 05/04/10 @ 06:08:26
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License: GNU General Public License (GPL) 2.0
Given an identifier for genome sequence (by default, genome of Mycoplasma genitalium: refseq:NC_000908) or raw sequence data in FASTA format, this workflow calculates and graphs the following properties using the G-language Genome Analysis Environment: GC skew (gcskew), cumulative GC skew (gcskew_cumulative), GC skew of coding/intergenic/GC3 (genomicskew), GC content with sliding windows (gcwin), replication origin and terminus (find_ori_ter), codon usage table (codon_usage), the Codon Adapta...
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Created: 21/07/10 @ 13:16:00 | Last updated: 21/07/10 @ 13:23:12
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve protein sequence and do a high speed BLAST and extract position from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example
accession : Q9NRA8
database : ddbjbct
program : tblastn
param : -b 100 -v 100
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Created: 21/07/10 @ 13:24:56 | Last updated: 01/09/10 @ 17:37:48
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve protein sequence and do a BLAST with options from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
exxample
accession : Q9NRA8
database : UNIPROT
program : blastp
param : -b 5 -m 7
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Created: 21/07/10 @ 13:56:08 | Last updated: 21/07/10 @ 13:58:41
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Attributions:
License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve nucleotide sequence and do a BLAST and extract position from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example :
accession : AB000100
database : DDBJ
program : blastn
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Created: 21/07/10 @ 14:08:32 | Last updated: 07/09/10 @ 10:04:55
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve nucleotide sequence and do a high speed BLAST and extract position from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example
accession : AB000100
database : DDBJ
program : blastn
param : -b 5 -m 7
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Created: 21/07/10 @ 14:21:50 | Last updated: 21/07/10 @ 14:21:52
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve nucleotide sequence and do a BLAST with options from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example
accession : AB000100
database : DDBJ
program : blastn
param : -b 5 -m 7
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Created: 21/07/10 @ 14:36:36 | Last updated: 01/09/10 @ 17:35:20
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve nucleotide sequence and do a VecScreen from DDBJ Web services : A system for quickly identifying segments of a nucleic acid sequence that may be of vector origin
informations on Web services available at http://xml.nig.ac.jp/index.html
accession : AB000100
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Created: 24/08/10 @ 13:45:14 | Last updated: 24/08/10 @ 13:45:16
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
With this workflow you can submit a BLAST query to the GPCRDB. Input requires a sequence with amino acids only.
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Created: 24/08/10 @ 13:58:32 | Last updated: 24/08/10 @ 13:58:35
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow allows you to create your own GPCR alignments. The alignments are built from the residues that are annotated with the general residue numbers. Alignments are therefore not built using standard alignment algorithms but are created by selecting residues that are likely to share the same position in the three-dimensional structure. Users can select the proteins and residue positions that should be aligned, allowing for the creation of e.g. an alignment of all binding pocket residue...
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Created: 10/03/10 @ 15:44:02 | Last updated: 10/03/10 @ 15:49:06
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow performs a generic protein sequence analysis. In order to do that a novel protein sequence enters into the software along with a list of known protein identifiers chosen by the biologist to perform a homology search, followed by a multiple sequence alignment and finally a phylogenetic analysis.
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Created: 01/09/10 @ 17:16:24 | Last updated: 01/09/10 @ 17:18:45
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Attributions:
License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve protein sequence and do a high speed BLAST from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example
accession : Q9NRA8
database : ddbjbct
program : tblastn
param : -b 100 -v 100
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Created: 01/09/10 @ 17:22:14 | Last updated: 01/09/10 @ 17:22:37
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Attributions:
License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve protein sequence and do a BLAST from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example
accession : Q9NRA8
database : UNIPROT
program : blastp
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Created: 01/09/10 @ 17:26:07 | Last updated: 01/09/10 @ 17:26:39
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve nucleotide sequence and do a high speed BLAST from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
accession : AB000100
database : DDBJ
program : blastn
param : -b 5 -m 7
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Created: 01/09/10 @ 17:31:29 | Last updated: 01/09/10 @ 17:33:51
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
retrieve nucleotide sequence and do a BLAST from DDBJ Web services
informations on Web services available at http://xml.nig.ac.jp/index.html
example :
accession : AB000100
database : DDBJ
program : blastn
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Created: 22/09/10 @ 12:40:55 | Last updated: 22/09/10 @ 13:35:26
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Blast Roche 454 sequences against a reference database on the Dutch Life Science Grid
Conversion of Roche 454 sequences to fasta
Blast sequences against a database
Parse Blast results
http://www.bioinformaticslaboratory.nl/
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Created: 22/09/10 @ 13:18:55 | Last updated: 22/09/10 @ 13:35:49
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Blat Roche 454 sequences against a reference database on the Dutch Life Science Grid
Conversion of Roche 454 sequences to fasta
Blat sequences against a database
http://www.bioinformaticslaboratory.nl/
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Created: 22/09/10 @ 13:22:57 | Last updated: 22/09/10 @ 13:36:12
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Blast and Blat Roche 454 sequences against a reference database on the Dutch Life Science Grid
Conversion of Roche 454 sequences to fasta
Blat sequences against a database
Blast sequences against a database
Parse Blast results
http://www.bioinformaticslaboratory.nl/
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Created: 17/01/11 @ 12:51:58 | Last updated: 17/01/11 @ 12:52:03
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow performs an NCBI blast at the EBI. It uses the new EBI services, which are asynchronous and require looping over the nested workflow (Status) until the workflow has finished. Many of the EBI services now work in this way, so you can use this workflow as an example of the invocation pattern and looping configuration.
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Created: 27/01/11 @ 17:03:03 | Last updated: 12/05/11 @ 13:31:41
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow retrieves all genes on human chromosome 22 that are associated with a disease and aligns the upstream regions with mouse and rat homologues. The alignments are plotted and corresponding sequence ids are also returned.Using Biomart and EMBOSS soaplab services, This workflow retrieves a number of sequences from 3 species: mouse, human, rat; align them, and returns a plot of the alignment result. Corresponding sequence ids are also returned.
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Created: 30/05/11 @ 01:20:35 | Last updated: 30/05/11 @ 01:26:47
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieve Protein or Genome sequences using the Distributed Annotation System (DAS).
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Created: 30/05/11 @ 03:12:23 | Last updated: 30/05/11 @ 03:14:03
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieve Protein or Genome sequences using the Distributed Annotation System (DAS) and create your own text output by modifying the JDAS component. To be able to use this workflow with JDAS, copy this file … http://www.ebi.ac.uk/~maven/m2repo/uk/ac/ebi/das/jdas/1.0.3/jdas-1.0.3.jar … to the lib folder inside the Taverna application. This jar file is a dependency needed to parse DAS outputs.
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Created: 01/06/11 @ 17:10:43 | Last updated: 01/06/11 @ 17:57:45
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieve Protein or Genome features (annotations) using the Distributed Annotation System (DAS).
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Created: 30/06/11 @ 15:26:13 | Last updated: 04/07/11 @ 16:51:41
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow carries out alignments using TCoffee and ClustalW2 for a set of non-redundant proteins where the starting point is a particular genomic coding sequence representing only one member of the gene family in a given species.
For the BioExtract Server implementation, the necessary steps for accomplishing this task involve:
1. Selecting the NCBI tblastx tool and providing the accession number of the known nucleotide sequence record as input.
2. The output from ...
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Created: 16/07/11 @ 19:08:38 | Last updated: 16/07/11 @ 19:35:11
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow performs multiple sequence similarity searches using the NCBI blast at the EBI. It uses the new EBI services, which are asynchronous and require looping over the nested workflow (Status) until the workflow has finished. Many of the EBI services now work in this way, so you can use this workflow as an example of the invocation pattern and looping configuration.
If you want to make a blast search for more than 10 sequences I would recommend you to run the workflow using the comm...
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Created: 19/08/11 @ 10:32:03 | Last updated: 19/08/11 @ 10:41:38
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
BLAST your sequences against the NucleaRDB. Input requires a sequence with amino acids only (no fasta format etc)
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Created: 13/08/10 @ 14:02:59 | Last updated: 19/11/10 @ 05:32:37
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License: GNU General Public License (GPL) 2.0
This workflow generates a sequence logo image for a set of amino acid sequences of FOXP2 gene, by downloading the amino acid sequences in Fasta format through Togo Web Service with UniProt identifiers (togoWS, provided by the G-language Genome Analysis Environment SOAP Service), running BLAST web service (runBLAST), retrieving a set of sequences from ID list (togoWS), aligning the sequence with MUSCLE (runMUSCLE), extracting a certain region from the alignment (extractalign, provided by Soapl...
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Created: 19/03/12 @ 11:59:57 | Last updated: 02/04/12 @ 22:51:40
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow retrieves a sequence associated with its features in embl format.
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Unique name: NextGenSeq Created: Monday 23 November 2009 @ 11:20:47 (GMT)
This group is for people wishing to contribute / find out more about 'Next Generation Sequencing' technology and data.
Please feel free to join this group and add any workflows you think others may benefit from.
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Created: 08/04/09 @ 17:53:46 | Last updated: 10/08/09 @ 12:30:59
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This spreadsheet contains information about the re-sequencing of the daxx gene, identified as a candidate QTg for the African Trypanosomiasis resiatance phenotype. This file is related to the paper: http://www.ncbi.nlm.nih.gov/pubmed/17709344
File type: Excel workbook Rating: 0.0 / 5 (0 ratings) | Comments: 0 | Viewed: 29 times | Downloaded: 21 times Tags: |
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