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Created: 07/04/09 @ 20:06:03 | Last updated: 06/12/10 @ 11:00:19
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License: Creative Commons Attribution 3.0 Unported License
Perform a ClustalW multiple sequence alignment using the EBI’s WSClustalW2 service (see http://www.ebi.ac.uk/Tools/webservices/services/clustalw2). The set of sequences to align are the input, the other parameters for the search (see Job_params) are allowed to default.
Note: the WSClustalW2 service used by this workflow is deprecated as of 21st September 2010 and should not be used in any new development. This service is will be retired during 2011. EBI's replacement ClustalW2 servi...
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Created: 07/04/09 @ 20:09:32
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License: Creative Commons Attribution 3.0 Unported License
Create a Neighbor-joining phylogenetic tree, with Kimura distance corrections, from a sequence alignment using the EBI's WSClustalW2 service (see http://www.ebi.ac.uk/Tools/webservices/services/clustalw2).
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Created: 31/05/08 @ 13:53:04 | Last updated: 03/12/10 @ 13:34:43
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License: Creative Commons Attribution 3.0 Unported License
Given a set of sequences perform an multiple sequence alignment and from the multiple alignment derive a phylogenetic tree. The popular ClustalW program (see http://www.clustal.org/), as implemented in the EBI's WSClustalW2 service (see http://www.ebi.ac.uk/Tools/webservices/services/clustalw2) is used to perform both tasks.
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Created: 07/04/09 @ 20:13:19
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License: Creative Commons Attribution 3.0 Unported License
An implmentation of the classical sequence analysis workflow:
Find homologues (sequence similarity search)
Fetch homologues
Align homologues (multiple sequence alignment)
Produce phylogenetic tree
In this implementation the EBI webservices are used:
WU-BLAST (WSWUBlast) blastp vs. UniProtKB
dbfetch (WSDbfetch)
ClustalW (WSClustalW2)
ClustalW (WSClustalW2)
Note: this version does not add the inital query sequence to the alignment, and so is most useful when used with the identifers...
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Created: 01/06/08 @ 12:20:29
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License: Creative Commons Attribution 3.0 Unported License
Transmembrane domain prediction using EMBOSS tmap with an input sequence alignment of homolouges:
Sequence similarity search (SSS) to find homologues
Fetch sequences of hits
Multiple sequence alignment (MSA) of hit sequences
EMBOSS tmap with alignment from 3.
Uses the EBI web services:
WSFasta (see http://www.ebi.ac.uk/Tools/webservices/services/fasta)
WSDbfetch (see http://www.ebi.ac.uk/Tools/webservices/services/dbfetch)
WSClustalW2 (see http://www.ebi.ac.uk/Tools/webservices/servic...
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Created: 10/03/10 @ 15:44:02 | Last updated: 10/03/10 @ 15:49:06
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow performs a generic protein sequence analysis. In order to do that a novel protein sequence enters into the software along with a list of known protein identifiers chosen by the biologist to perform a homology search, followed by a multiple sequence alignment and finally a phylogenetic analysis.
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Created: 06/12/10 @ 13:16:52
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License: Creative Commons Attribution 3.0 Unported License
Perform a ClustalW2 alignment using the EMBL-EBI’s ClustalW2 (SOAP) service (see http://www.ebi.ac.uk/Tools/webservices/services/msa/clustalw2_soap).
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Created: 07/08/09 @ 16:04:23 | Last updated: 07/08/09 @ 16:23:53
A tutorial to demonstrate how to create a workflow within the BioExtract Server at bioextract.org. Specifically, it shows how to perform a phylogenetic analysis on a set of proteins where the starting point is a query for specific nucleotide gene sequences.
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Created: 07/08/09 @ 16:20:06 | Last updated: 07/08/09 @ 16:25:46
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This tutorial demonstrates how to create a workflow within the BioExtract Server at bioextract.org. Specifically, it shows how to perform a phylogenetic analysis on a set of proteins where the starting point is a query for specific nucleotide gene sequences.
File type: Word 2007 document Rating: 0.0 / 5 (0 ratings) | Comments: 0 | Viewed: 29 times | Downloaded: 25 times Tags: |
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