Carol Lushbough is an Assistant Professor of Computer Science at the University of South Dakota.
Her primary teaching areas include Systems Analysis, Software Engineering, and Bioinformatics.
In addition to her teaching responsibilities, she collaborates with Dr. Volker Brendel from
Iowa State University on the NSF funded project "Cyberinfrastructuer for (Comparative) Plant Genome
Research through PlangGDB". Her main contributes to the project is the development of the
BioExtract Server (http://bioextract.org). The BioExtract Server is a Web-based data integration
application designed to consolidate, analyze, and serve data from heterogeneous biomolecular
databases in the form of a mash-up. The basic operations of the BioExtract Server allow researchers,
via their Web browsers, to: specify data sources, flexibly query data sources, apply analytic tools,
download result sets, and store query results for later reuse. As a researcher works with the system,
their “steps” are saved in the background. At any time these steps can be preserved long-term as a
workflow simply by providing a workflow name and description.
Other contact details:
University of South Dakota
Interests:
Bioinformatics
Data Integration
Scientific Workflows
Field/Industry:
Computer Science
Occupation/Role(s):
Assistant Professor of Computer Science
Organisation(s):
University of South Dakota
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tblastx non-redundant alignment
(v1)
Created: 30/06/11 @ 15:26:13
| Last updated: 04/07/11 @ 16:51:41
Credits:
Carol Lushbough
License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow carries out alignments using TCoffee and ClustalW2 for a set of non-redundant proteins where the starting point is a particular genomic coding sequence representing only one member of the gene family in a given species.
For the BioExtract Server implementation, the necessary steps for accomplishing this task involve:
1. Selecting the NCBI tblastx tool and providing the accession number of the known nucleotide sequence record as input.
2. The output from ...
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Sequence Format Conversion
(v1)
Created: 28/06/11 @ 20:52:07
| Last updated: 28/06/11 @ 21:03:58
Credits:
Carol Lushbough
License: Creative Commons Attribution-Share Alike 3.0 Unported License
Demonstrate the use of the format conversion tool. Converts a DNA sequence in fasta format into plain, genbank, and phylip formats.
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Multiple Protein Alignment Profiling(1)
(v4)
Created: 13/01/10 @ 18:52:45
| Last updated: 17/11/10 @ 14:13:20
Credits:
Carol Lushbough
Alan Williams
Attributions:
A workflow version of the EMBOSS tutorial
License: Creative Commons Attribution-Share Alike 3.0 Unported License
Designed from an example workflow at Traverna, this workflow retrieves a set of sequences from UniProt, then simultaneously scans for transmembrane regions and performs a multiple alignment using EMMA. The alignment is then plotted to a set of PNG images, followed by a profile analysis using prophecy and prophet tools.
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Phylogeny Bootstrapped Algorithm
(v2)
Created: 13/01/10 @ 18:47:06
| Last updated: 17/11/10 @ 13:57:19
Credits:
Carol Lushbough
License: Creative Commons Attribution-Share Alike 3.0 Unported License
Bootstrapped sequences algorithm reads in a data set, and produces multiple data sets from it by bootstrap resampling. http://pro.genomics.purdue.edu/cgi-bin/emboss/help/fseqboot
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Liliopsida Protein Alignment
(v6)
Created: 13/01/10 @ 18:42:35
| Last updated: 17/11/10 @ 13:46:04
Credits:
Carol Lushbough
License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow retrieves Liliopsida chloroplast petb gene sequences from NCBI Nucleotide, removes duplicate sequences and saves the results at BioExtract Server. These results are then converted into GenBank format and fed into Fetch Translation, which removes the translation from the CDS coding region. Translations are then used to build a multiple alignment using ClustalW.
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Nucleotide InterProScan for the BioExtract Server
(v3)
Created: 16/04/09 @ 15:26:57
| Last updated: 01/07/09 @ 08:32:34
Credits:
Carol Lushbough
Hamish McWilliam
Attributions:
Nucleotide_InterProScan
License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow can be downloaded and imported into the BioExtract Server at bioextract.org.
This workflow is a BioExtract Server process similar to the Nucleotide InterProScan workflow designed and implement in Taverna by Hamish McWilliams.
The InterProScan tool (http://www.ebi.ac.uk/Tools/InterProScan/) searches a protein sequence against a selection of protein domain, feature and family signature databases, and integrates the results giving potential assignments to InterPro entries and Gen...
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