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Created: 01/06/08 @ 12:20:29
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License: Creative Commons Attribution 3.0 Unported License
Transmembrane domain prediction using EMBOSS tmap with an input sequence alignment of homolouges:
Sequence similarity search (SSS) to find homologues
Fetch sequences of hits
Multiple sequence alignment (MSA) of hit sequences
EMBOSS tmap with alignment from 3.
Uses the EBI web services:
WSFasta (see http://www.ebi.ac.uk/Tools/webservices/services/fasta)
WSDbfetch (see http://www.ebi.ac.uk/Tools/webservices/services/dbfetch)
WSClustalW2 (see http://www.ebi.ac.uk/Tools/webservices/servic...
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Created: 30/05/08 @ 23:50:46 | Last updated: 06/12/10 @ 10:52:25
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License: Creative Commons Attribution 3.0 Unported License
Run a FASTA or SSEARCH sequence similarity search using the EBI's WSFasta service (see http://www.ebi.ac.uk/Tools/webservices/services/fasta).
Note: the WSFasta service used by this workflow is deprecated as of 21st September 2010 and should not be used in any new development. This service is will be retired during 2011. EBI's replacement FASTA services (REST or SOAP) should be used instead.
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Created: 30/05/08 @ 23:53:16 | Last updated: 06/12/10 @ 10:53:23
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License: Creative Commons Attribution 3.0 Unported License
Run a FASTA analysis using the EBI’s WSFasta service (see http://www.ebi.ac.uk/Tools/webservices/services/fasta). This workflow wraps the EBI_FASTA workflow to provide a basic user interface which prompts for the required inputs: sequence, database, FASTA program and user e-mail. Other parameters (e.g. matrix, gap penalties, etc.) are allowed to default.
Note: the WSFasta service used by this workflow is deprecated as of 21st September 2010 and should not be used in any new developme...
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Created: 09/11/07 @ 10:41:12 | Last updated: 05/06/08 @ 15:18:07
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieves a protein sequence in Fasta format from Genbank and then performs a BLAST on that sequence
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Created: 09/03/09 @ 11:38:10
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow queries Biomart to retrieve the Ensembl gene id, protein id, gene name, description and amino acid sequence from the Ensembl Homo sapiens dataset. The user needs to specify a defined chromosomal region i.e. Chromo = 1, Start = 100000000, End = 250000000. This returns all unique entries in FASTA format.
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Created: 20/03/09 @ 11:22:45 | Last updated: 20/03/09 @ 11:29:16
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This work flow is designed to take an EMBL file containing the genomic data for an identified bacterium. From this information the workflow can determine whether or not that this strain is an MRSA type of bug. This can be determined based on the MecA profile of the given strain.
Blast is utilised to find a relationship with given proteins and that of know S. aureus strains. This phylogenic output is generated from a ClustalW algorithm that plots a phylogenic tree.
The output is prese...
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Created: 20/10/09 @ 17:00:59
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Search in the Datenbank SWISS the Sequence 1220173
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Created: 16/11/09 @ 12:37:25
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Ex of exercise
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Created: 19/03/10 @ 13:16:24 | Last updated: 19/03/10 @ 13:33:53
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Takes GI number for source (non-pathogenic) and target (pathogneic) genomes, extracts list of all proteins from each genome using GenBank database. Outputs prtoeins in FastA format. Creates database from source proteins using formatdb (locally installed) and blasts (local installed) proteins from target against this database. Extracts protens which are unique (no blast hits) to the target (pathogenic) genome based on eValue set by user. Takes unique proteins from target and blasts aga...
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Created: 19/03/10 @ 15:17:53
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Retrieves the genome seqn for both the target and source strains using gi nos
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Created: 05/04/10 @ 06:08:25 | Last updated: 05/04/10 @ 06:08:26
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License: GNU General Public License (GPL) 2.0
Given an identifier for genome sequence (by default, genome of Mycoplasma genitalium: refseq:NC_000908) or raw sequence data in FASTA format, this workflow calculates and graphs the following properties using the G-language Genome Analysis Environment: GC skew (gcskew), cumulative GC skew (gcskew_cumulative), GC skew of coding/intergenic/GC3 (genomicskew), GC content with sliding windows (gcwin), replication origin and terminus (find_ori_ter), codon usage table (codon_usage), the Codon Adapta...
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Created: 24/03/11 @ 19:49:43 | Last updated: 01/04/11 @ 12:26:27
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
The workflow parses uses the tab-delimited BLAST results to determine the unique proteins found in the target genome that have no similarity to the source genome.The workflow parses uses the blast results to determine the unique proteins found in the target genome that have no similairty to the source genome. Using these unique protein ids, and the original target protein fasta file, a fasta file of unique proteins is created.This workflow allows you to configure a BioMart query to fetch sequ...
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Created: 25/03/11 @ 20:06:13 | Last updated: 01/04/11 @ 12:40:56
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
The drug repurposing workflow system screens at least 20 bacterial proteomes against this set of proteins that are already being treated against using established drugs. By screening the bacterial proteomes it will be possible to find proteins of highly similar structure to those that are existing drug protein targets and so this will infer that it is highly likely that the drugs can be used as antimicrobials against these proteins of highly similar structure. Proteomes that will be screene...
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Created: 30/03/11 @ 10:34:37 | Last updated: 30/03/11 @ 10:34:40
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
Uses a tab-delimited file with protein target and drug information (created in Ondex) to compile a protein FASTA file including each target.
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Created: 16/07/11 @ 19:08:38 | Last updated: 16/07/11 @ 19:35:11
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow performs multiple sequence similarity searches using the NCBI blast at the EBI. It uses the new EBI services, which are asynchronous and require looping over the nested workflow (Status) until the workflow has finished. Many of the EBI services now work in this way, so you can use this workflow as an example of the invocation pattern and looping configuration.
If you want to make a blast search for more than 10 sequences I would recommend you to run the workflow using the comm...
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Created: 13/08/10 @ 14:02:59 | Last updated: 19/11/10 @ 05:32:37
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License: GNU General Public License (GPL) 2.0
This workflow generates a sequence logo image for a set of amino acid sequences of FOXP2 gene, by downloading the amino acid sequences in Fasta format through Togo Web Service with UniProt identifiers (togoWS, provided by the G-language Genome Analysis Environment SOAP Service), running BLAST web service (runBLAST), retrieving a set of sequences from ID list (togoWS), aligning the sequence with MUSCLE (runMUSCLE), extracting a certain region from the alignment (extractalign, provided by Soapl...
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