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Created: 13/03/08 @ 14:38:03 | Last updated: 16/02/09 @ 16:41:29
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
The AffyArrayQualityAnalysis web services provide quality control for raw Affymetrix GeneChip data. They are wrappers around Philip de Groot's quality control R script to provide remote programmatic access. This example workflow demonstrates the use of the AffyArrayQualityAnalysis services. The flow is as follows:
A client executes the AffyArrayQualityAnalysis_submit service with two inputs: a User object and a collection of URLs linking to CEL files.
The User object contains a u...
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Created: 16/02/09 @ 16:32:36 | Last updated: 16/02/09 @ 16:42:10
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
The AffyArrayNormalization web services normalise raw Affymetrix GeneChip data. They are wrappers around Philip de Groot's normalization R script to provide remote programmatic access. This example workflow demonstrates the use of the AffyArrayNormalization services. The flow is as follows:
A client executes the AffyArrayNormalization_submit service with two inputs: a User object and a collection of URLs linking to CEL files.
The User object contains a user ID, a password and an ...
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Created: 03/10/07 @ 18:36:09 | Last updated: 22/11/07 @ 16:03:15
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License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
This is a revised workflow for the Graves disease scenario gene annotation pipeline used in the myGrid project. The workflow had to be re-written due to the loss of the services invoked in the original workflow.
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Created: 03/10/07 @ 18:35:55 | Last updated: 26/11/09 @ 17:34:24 License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
This workflow takes in a CEL file and a normalisation method then returns a series of images/graphs which represent the same output obtained using the MADAT software package (MicroArray Data Analysis Tool) [http://www.bioinf.manchester.ac.uk/MADAT/index.html]. Also returned by this workflow are a list of the top differentialy expressed genes (size dependant on the number specified as input - geneNumber), which are then used to find the candidate pathways which may be influencing the observed ...
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Created: 08/02/08 @ 14:17:23 | Last updated: 13/02/09 @ 11:29:52
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow takes in a CEL file and a normalisation method then returns a series of images/graphs which represent the same output obtained using the MADAT software package (MicroArray Data Analysis Tool) http://www.bioinf.manchester.ac.uk/MADAT/index.html. Also retruned by this workflow are a list of the top differentialy expressed genes (size dependant on the number specified as input - geneNumber), which are then used to find the candidate pathways which may be influencing the observed ch...
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Created: 08/02/08 @ 14:30:17 | Last updated: 03/12/09 @ 16:50:29
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow takes in probesets from and AffyMetrix Affy HG U133A micorarray experiment and returns: genes ; gene start and end positions; chromosome where genes reside; ensembl trasncripts; SwissProt ids. The final output of the workflow is a list of candidate pathways which are linked to the genes expressed in the microarray data.
Example input for this workflow is:
212283_at
221634_at
220399_at
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Created: 17/02/09 @ 04:40:17 | Last updated: 17/02/09 @ 04:42:28
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow initialize the Sesame triplestore with initial data. The 5 rdfiser in JSP are needed, they are executed on localhost.
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Created: 17/02/09 @ 04:54:30
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
The affymetrix data source is not public.
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Created: 06/03/09 @ 17:18:13 | Last updated: 03/12/09 @ 16:59:11
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License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
This workflow searches for genes obtained from affy_ath1 affymetrix probeset identifiers. The Entrez and UniProt identifiers are then sent to KEGG to obtain KEGG gene identifiers. The KEGG gene identifiers are then used to searcg for pathways in the KEGG pathway database.
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Created: 08/07/09 @ 16:28:34
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License: Creative Commons Attribution-No Derivative Works 3.0 Unported License
This workflow searches for genes which are found to be differentially expressed in a microarray study using Arabidopsis thaliana. The workflow requires an input of a list of differentially expressed AffyMetrix Probeset identifiers. Data is then extracted from BioMart to annotate each of the genes. The UniProt identifiers are then sent to KEGG to obtain KEGG gene identifiers. The KEGG gene identifiers are then used to searcg for pathways in the KEGG pathway database.
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Created: 15/11/10 @ 12:25:01 | Last updated: 15/11/10 @ 12:28:12
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow searches for genes which were found to be differentially expressed from a microarray study in the mouse, Mus musculus. The workflow requires an input of gene ref_seq identifiers. Data is then extracted from BioMart to annotate each of the genes found for each ref_seq id. The Entrez and UniProt identifiers are then sent to KEGG to obtain KEGG gene identifiers. The KEGG gene identifiers are then used to search for pathways in the KEGG pathway database.
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Created: 15/11/10 @ 12:30:21 | Last updated: 15/11/10 @ 12:30:59
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This workflow takes in two lists of KEGG pathway ids. These are designed to come from pathways found from genes in a QTL (Quantitative Trait Loci) region, and from pathways found from genes differentially expressed in a microarray study. By identifying the intersecting pathways from both studies, a more informative picture is obtained of the candidate processes involved in the expression of a phenotype
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Created: 28/04/11 @ 14:15:33 | Last updated: 13/12/11 @ 16:00:25
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
No description
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Created: 28/04/11 @ 14:12:33 | Last updated: 13/12/11 @ 16:00:52
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
No description
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Created: 04/05/11 @ 14:40:17 | Last updated: 13/12/11 @ 16:01:13
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
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Created: 08/04/09 @ 13:14:54 | Last updated: 11/08/09 @ 14:50:01
It is increasingly common to combine Microarray and Quantitative Trait Loci data to aid the search for candidate genes responsible for phenotypic variation. Workflows provide a means of systematically processing large datasets, and represent a framework for the re-use and the explicit declaration of experimental methods. In this pack is a paper which describes the issues facing the manual analysis of microarray and QTL data for the discovery of candidate genes underlying complex phenotypes.&n...
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Created: 11/08/09 @ 14:44:16 | Last updated: 11/08/09 @ 14:52:12
This pack is for investigating links between the genotype of an organisms to possible pathways. This constitutes half of the pathway-driven approach, genotype to pathway, and pathway to phenotype.
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Created: 08/04/09 @ 13:27:20
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This protocol provides details on how to identify candidate genes from the returned workflow results.
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Created: 08/04/09 @ 17:57:57
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This spreadhseet contains all of the intersecting pathways between the Tir1 QTL and day 7 differentially expressed genes
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Created: 08/04/09 @ 19:30:15 | Last updated: 10/08/09 @ 11:05:59
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License: Creative Commons Attribution-Share Alike 3.0 Unported License
This file contains all genes identified in the Tir1 QTL region.
Each gene is (if available) associated with an Entrez, UniProt, External Gene Id, Affymetrix probeset identifers (mouse_430_A chip), and Ensembl identifers for genes and their transcripts.
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Created: 01/04/11 @ 09:08:45 | Last updated: 01/04/11 @ 09:08:51 License: Creative Commons Attribution-Share Alike 3.0 Unported License
This file contains a list or affymetrix probest identifiers for Human gene expression data relating to the: Impact of NRSF variant over-expression in SK-N-AS human neuroblastoma cells (http://www.ebi.ac.uk/arrayexpress/browse.html?keywords=E-GEOD-22467&expandefo=on)
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Created: 01/04/11 @ 09:12:28 | Last updated: 01/04/11 @ 09:12:40
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Attributions: License: Creative Commons Attribution-Share Alike 3.0 Unported License
This file contains a list processed for Human gene expression CEL files relating to the: Impact of NRSF variant over-expression in SK-N-AS human neuroblastoma cells (http://www.ebi.ac.uk/arrayexpress/browse.html?keywords=E-GEOD-22467&expandefo=on).
The CEL files were analysed using the MADAT Taverna workflow available on myExperiment:
http://www.myexperiment.org/workflows/2002.html
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